• Proposal: 10/31/2019


Single Cell HbA1C Fraction Distribution Assay

This RFP aims at funding an analytical approach to precisely measure percent glycated hemoglobin (HbA1C) fraction at the single Red Blood Cell (RBC) level from whole-blood samples.  These measures should enable single cell HbA1C fraction dynamics to be expressed from a bulk blood sample and be repeatable to a high-degree of precision/accuracy within and between laboratories and validated.

This request for proposal is asking for a research team to develop a new flow cytometric or any other (e.g., mass spectroscopy) approach that will allow for the calculation of percent glycated hemoglobin on a single cell level and could eventually be transferable to anti-doping laboratories.  This measurement would likely have many clinical benefits for patients and clinicians, diabetic researchers, and the sport anti-doping community.

The analytical approach must be able to measure HbA1c in hundreds of RBCs or more from each blood sample and eventually be transferrable for routine use in anti-doping laboratories.   Proposals must include validation studies demonstrating the precision of the method’s inference of all deciles of the single-RBC HbA1c distribution, i.e, what is the analytic variation for the 10th percentile of the single-RBC HbA1c distribution, the 20th percentile, etc.  The mean of the single-RBC HbA1c distribution should be validated against a validated HbA1c assay.

Eligibility & Submission Requirements

Applicants should have expertise in the fields of the appropriate clinical laboratory methodology, HbA1c, bioinformatics, and biomarker discovery.  Applicants should have access to blood samples derived from a diabetic population or from clinical studies involving significant hematologic perturbations such as blood removal or infusion.

Collaborations between research groups is encouraged.

Applicants are encouraged to contact the Partnership for Clean Competition for assistance with collaborations and with regard to technical aspects of this project.

The successful candidate must be willing to publish their research findings in a peer-reviewed journal.

Contact Information


Michael Pearlmutter