Matt Dubach

Matt Dubach

Department: Bioengineering
Advisor: Heather Clark



My research focuses on developing novel fluorescent nanoparticles to use as tools for measurement of chemical concentrations in biological samples. With these nanoparticles, we have measured sodium dynamics in excitable cells and sodium concentrations in the skin of mice, which correlate to blood concentrations. I am currently creating nanoparticle sensors that will function as a long term sensing tattoo in the skin and developing novel imaging devices to measure the sensor response.

This research has enabled me to establish a company, Ionu Biosystems, with the goal of making our nanoparticle sensing technology a medical product for human use. We recently received an SBIR grant from NASA to develop a smart phone Chem8 blood test. As the principal investigator on this grant I plan to utilize the skills and knowledge I obtained in the Igert nanomedicine program to advance our product towards human use.



  • Smart Phone Optical Diagnostics, Dubach JM, Point of Care Diagnostics, Molecular Med Tri Con, Feb 2012
  • Optical sensors for in vivo monitoring of ions and small molecules., Dubach JM, et al, Nanotech, Jun 2011
  • Miniemulsion for Pasive Cellular Loading of Fluorescent Ion Detecting Nanodroplets, Dubach JM, Materials Research Society, Nov 2010
  • Nanosensors for the Measurement of Sodium Flux During Action Potentials, Dubach JM, Pittcon
  • Novel Intracellular Sodium Nanosensors to Study Sodium Dynamics in Cardiomyocytes, Dubach JM, American Heart Association
  • Sodium Nanosensors Fluorescently Monitor Sodium Dynamics in Isolated Cardiomyocytes, Dubach JM, Pittcon
  • Myocyte Ion Dymanics during Action Potentials Measured by Fluorescent Nanosensors, Dubach JM, Heart Rhythm
  • Optical Nanosensors for Imaging Sodium Dynamics during Action Potential Propagation, Dubach JM, Pittcon
  • Intracellular Sodium Flux Measured with Fluorescent Sodium Sensors, Dubach JM, Gordon Research Seminar: Analytical Biosensors


  • In vivo sodium concentration continuously monitored with fluorescent sensors, Dubach JM, Lim E, Zhang N, Francis KP, Clark HA, Int Biol, V.3 (2), P. 142 – 148 (2011)
  • Optimization of Fluorescent Optodes for In Vivo Glucose Monitoring, Balaconis, M.K., Billingsley, K., Dubach, J.M., Cash, K.J., Clark, H.A., Journal of Diabetes Science and Technology, V.5(1), P.1650 (2011)
  • Microworm Optode Sensors Limit Particle Diffusion to Enable In Vivo Measurements, Ozaydin-Ince G*, Dubach JM*,Gleason KK,Clark HA, PNAS, V.108(7), P.2656 (2011)
  • Fluorescent nanoparticles for the measurement of ion concentrations in biological systems, Dubach J.M., Balaconis M.K., Clark H.A., J. Vis. Exp. , V.53, P.e2896 (2011)
  • Ion-Selective-Optodes Measure Extracellular Potassium Flux in Excitable Cells, Harjes DI, Dubach JM, Das S, Rosenzweig A, Clark HA, Macromolecular Rap Com, V.31, P.217 (2010)
  • Monochloromine-induced toxidicy and dysregulation of intracellular Zn2+ in parietal cells of rabbit gastric glands, Kohler J, Dubach JM, Naik H, Tai K, Blass A, Soybel DI, Am J Phys Gastrointest Liver Physiol, V.299(1), P.G170-8 (2010)
  • Fluorescent Nano-Optodes for Glucose Detection, Billingsley K., Balaconis M.K., Dubach J.M., Zhang N., Lim E., Francis K.P., Clark H.A., Anal Chem, V.82(9), P.3703 (2010)
  • Visualizing sodium sparks in isolated cardiomyocytes using fluorescent nanosensors, Dubach JM, Das S, Rosenzweig A, Clark HA, PNAS, V.106(38), P.16145 (2009)
  • Protection of sensors for biological applications by photoinitiated chemical vapor deposition of hydrogel thin films, Baxamusa SH, Montero L, Dubach JM, Clark HA, Borros S, Gleason KK, Biomacromolecules, V.9(10), P.2857-62 (2008)
  • Thiol-Oxidant Monochloramine Mobilizes Intracellular Ca2+ in Parietal Cells of Rabbit Gastric Glands, Walsh BM, Naik HB, Dubach JM, Beshire M, Wieland AM, Soybel DI, Am J Physiol Cell Physiol, V.293(5), P.C1687-97 (2007)
  • Fluorescent ion-selective nanosensors for intracellular analysis with improved lifetime and size, Dubach JM, Harjes DI, Clark HA, Nano Lett, V.7(6), P.1827-31 (2007)
  • Ion-Selective Nano-optodes Incorporating Quantum Dots, Dubach JM, Harjes DI, Clark HA, J Am Chem Soc, V.129(27), P.8418-9 (2007)
  • Intracellular Ca(2+) and Zn(2+) signals during monochloramine-induced oxidative stress in isolated rat colon crypts, Cima RR, Dubach JM, Wieland AM, Walsh BM, Soybel DI., Am J Physiol Gastrointest Liver Physiol, V.290(2), P.G250-61 (2006)