Agnes Rafalko

Agnes Rafalko

Department: Chemistry
Advisor: William Hancock

Collaborators: Mansoor Amiji



Agnes Rafalko works for the Barnett Institute of Chemical & Biological Analysis. She is developing new analytical/mass spectrometry based platforms for the study of plasma in disease. Her focus is clinical proteomics, the search for and validation of biomarkers that predict the progress of disease and can be used to segregate patients for individualized therapy.

Agnes grew up in a small town in eastern part of Poland. Agnes graduated with a BS in Biology/Chemistry form Saint Joseph College, CT.



  • IEF-Chip/HPLC-Chip-SRM-MS: Quantification of Proteins Using Chip-Based Enrichment/Separation with Selected Reaction Monitoring MS and Stable Isotope Dilution, Rafalko, A., Dai, S., Hancock, W., Karger, B.L. and Hincapie M., 58th ASMS Conference on Mass Spectrometry conference, Salt Lake City, Utah., May 2010
  • Lectin-Gold Nanoparticle Conjugates for Imaging of Cell Surface Oligosaccharides for Early Diagnosis of Cancer, Rafalko, A., Hancock, W., Amiji, M.M. and Hincapie M., 2010 IGERT Annual Principal Investigators Meeting, Washington D.C., May 2010
  • Immunoaffinity Enrichment and Targeted Mass Spectrometry for Quantification of Carbonic Anhydrase 12 Protein as a Biomarker for Renal Cell Carcinoma, Agnes Rafalko, 5th Annual USHUPO Conference, San Diego, CA, Feb 2009


  • Immunoaffinity enrichment and multiple reaction monitoring mass spectrometry (MRM-MS) for detection of Carbonic Anhydrase 12 (CA12) protein in cultured renal carcinoma cells, Agnes Rafalko, Othon Iliopoulos, Christopher Neil, Vincent A. Fusaro, William Hancock, Marina Hincapie, Submitted to Journal of Proteome Research, V., P. (2009)