It is becoming increasingly clear that characterization of the protein ensemble – the collection of all conformations of which the protein is capable – will be a critical step in developing a full understanding of the linkage between structure, dynamics and function. X-ray solution scattering in the small angle (SAXS) and wide-angle (WAXS) regimes represents an important new window to exploring the behavior of ensembles. The characteristics of the ensemble express themselves in x-ray solution scattering data in predictable ways. We have collected and analyzed WAXS data on a range of biomolecular systems and demonstrated the modulation of these ensembles by ligand binding; mutation and environmental factors.
Makowski, L., D. J. Rodi, S. Mandava, D. Minh, . D. Gore and R. F. Fischetti (2008) Molecular crowding inhibits intramolecular breathing motions in proteins. J. Mol. Biol. 375, 529-546. PMID: 18031757
Makowski, L. D. Gore, S. Mandava, D. Minh, S. Park, D. J. Rodi and R. F. Fischetti (2011) X-ray solution scattering studies of the structural diversity intrinsic to protein ensembles (submitted for publication).
Yang, S., L. Blachowicz, L. Makowski and B. Roux (2010) Multidomain assembled States of Hck Tyrosine Kinase in Solution. Proc. Natl. Acad. Sci. 107, 15757-62. PMID: 20798061
See also the News and Views article on this work in Nature: B. Pau and M. Blackledge (2010) Proteins in Dynamic Equilibrium. Nature 468, 1046-1048.See also the News and Views article on this work in Nature: B. Pau and M. Blackledge (2010) Proteins in Dynamic Equilibrium. Nature 468, 1046-1048.