Stem cell differentiation under specified Stress field on shaft loaded blister setup. The developmental versatility of embryonic stem (ES) cells offers a powerful approach for directing cell fate and is a promising source of progenitors for cell replacement therapy and tissue regeneration. ES cells can differentiate into a wide spectrum of cell types, such as cardiomyocytes and endothelial cells, by forming embryoid bodies (EBs). Those lineages arise from distinct mesoderm subpopulations that develop sequentially from premesoderm cells. Such lineage specification is highly coordinated with differential changes in gene expression. Despite the therapeutic potential of ES cells, one of the significant challenges to their widespread clinical use, is the inability to homogeneously direct ES cell differentiation into specific lineages. We have developed an experimental and theoretical model for shaft loaded blister setup which allow us to use those models and results as a template for studying the effect of stress on stem cell differentiation.