Differentiation Capabilities Using Suspension-based Shear Exposure for Undifferentiated Adult Human Mesenchymal Stem Cells

Abstract

Shear stimulation of adult human mesenchymal stem cells (hMSCs) has been well studied through various inquiries using plated systems. Using a microfluidic approach of varied shear exposure time in a suspension-based system, the differentiation capabilities of hMSCs were studied. Previous work has failed to investigate the differentiation properties of hMSCs in a more in vivo-like environment. Without the use of a bioreactor or a specific media formulation, hMSCs were exposed to differing times of shear exposure via flow through a tubing system. Flow cytometry and qRTPCR analysis techniques were used to study how the suspension-based flow caused the hMSCs to differentiate towards different lineages. This study demonstrates how flow through a tube can differentiate hMSCs and reduce the time and costs currently required to differentiate hMSCs towards adipogenic and muscle lineages.