Shear stimulation of adult human mesenchymal stem cells (hMSCs) has been well studied through various inquiries using plated systems. Using a microfluidic approach of varied shear exposure time in a suspension-based system, the differentiation capabilities of hMSCs were studied. Previous work has failed to investigate the differentiation properties of hMSCs in a more in vivo-like environment. Without the use of a bioreactor or a specific media formulation, hMSCs were exposed to differing times of shear exposure via flow through a tubing system. Flow cytometry and qRTPCR analysis techniques were used to study how the suspension-based flow caused the hMSCs to differentiate towards different lineages. This study demonstrates how flow through a tube can differentiate hMSCs and reduce the time and costs currently required to differentiate hMSCs towards adipogenic and muscle lineages.