Dr. Nathalie Agar.  Assistant Professor of Neurosurgery, Assistant Professor of Radiology, Harvard Medical School Department of Neurosurgery Brigham and Women’s Hospital Department of Cancer BiologyDana-Farber Cancer Institute Boston, MA 02115

Mass spectrometry provides a new tool for the direct imaging of tissue during neurosurgery, and can also provide significant insight in the development of drugs targeting tumors of the central nervous system. Using desorption electrospray ionization mass spectrometry (DESI MS), we rapidly detect tumor metabolites from tissue sections of surgically-resected gliomas without complex or time-consuming preparation.The method was validated by correlating 2D mass spectrometry imaging of glioma specimens with histopathology, and used to detect tumor tissue within seconds to minutes. Imaging tissue sections with DESI MS shows that diagnostic molecular signatures overlap with areas of tumor, thereby indicating tumor margins. We have installed a mass spectrometer in our Advanced Multimodality Image Guided Operating (AMIGO) suite at BWH and demonstrate the molecular analysis of surgical tissue during brain surgery.

Dr. Nathalie Agar seminar abstract