J. Timothy Sage

Experimental Biological Physics

J. Timothy Sage

Associate Professor
PhD University of Illinois at Urbana-Champaign, 1986

Research Summary:

Professor Sage’s research is motivated by a fascination with the physical basis for the function of proteins. He develops and applies novel spectroscopic approaches to understand the structure, dynamics, and function of biological macromolecules. Infrared portein crystallography uses polarized infrared measurements on oriented protein crystals to identify reaction intermediates and refine the structure of protein active sites. A new synchrotron-based technique, nuclear resonance vibrational and reactive dynamics of iron in these complex molecules.

Recent Publications:

Quantitative Vibrational Dynamics of Iron in Carbonyl Porphyrins, B. M. Leu, N. J. Silvernail, M. Z. Zgierski, G. R. A. Wyllie, M. K. Ellison, W. R. Scheidt, J. Zhao, W. Sturhahn, E. E. Alp, J. T. Sage, Biophys. J. 92, 3764-3783 (2007).

Charge Transfer in Green Fluorescent Protein, J. J. van Thor, J. T. Sage, Photochem. Photobiol. Sci. 5, 597-602 (2006).

Ultrafast and Low Barrier Motions in the Photoreactions of the Green Fluorescent Protein, J. J. van Thor, G. Y. Georgiev, M. Towrie, J. T. Sage, J. Biol. Chem. 280, 33652–33659 (2005).

Direct Probe of Iron Vibrations Elucidates NO Activation of Heme Proteins, W. Zeng, N. J. Silvernail, D. C. Wharton, G. Y. Georgiev, B. M. Leu, W. R. Scheidt, J. Zhao, W. Sturhahn, E. E. Alp, J. T. Sage, J. Am. Chem. Soc. 127, 11200-11201 (2005).

Nuclear Resonance Vibrational Spectroscopy–NRVS, W. R. Scheidt, S. M. Durbin, J. T. Sage, J. Inorg. Biochem. 99, 60-71 (2005).