Structural Chromosome Maintenance in Bacteria

When: Thursday, November 29, 2012 at 4:00 pm
Where: DA 114
Speaker: Valentin V. Rybenkov
Organization: University of Oklahoma
Sponsor: Physics Colloquium

 “Across kingdoms of life, the global folding of the chromosome requires the activity of condensins. These characteristically V-shaped multisubunit cellular ATPases establish the giant loop organization of chromosome. Using a combination of biochemistry and single DNA nanomanipulations, we explored DNA organization by the Escherichia coli condensin MukBEF. We show that MukBEF acts as an ATP modulated macromolecular assembly that can bring distant DNAs into a stable complex. The resulting activity offers a biochemical mechanism for self-assembly of the chromosome scaffold and suggests that MukBEF forms a network of loosely linked clamps that divides the chromosome into a set of giant loops. We discuss contribution of condensins to chromosome segregation during cell division. Finally, we describe a novel family of condensins, MksBEF, and show that condensins play versatile roles in environmental and pathogenic bacteria.”