Architectural role of HMO1 in Bending, Bridging and Packaging DNA

When: Wednesday, March 20, 2013 at 12:00 pm
Where: DA 114
Speaker: Divakaran Murugesapillai
Organization: Northeastern University
Sponsor: Introduction to Physics Research

To characterize DNA binding we combine single molecule stretching experiments and AFM imaging upon the HMGB protein HMO1. By stretching DNA bound to HMO1, we determine the dissociation constant Kd and measure protein induced bending angles.To further investigate the nature of these bends, AFM images of HMO-DNA complexes are imaged to probe the behavior of these complexes as a function of protein concentration.