biotechnology research

The use of nano-liter reaction volumes and parallel sample processing offered by microfluidic devices make them ideally suited to total chemical and bioassay analysis, ultra-high throughput screening applications, and other cases where samples and reagents are available in limited quantities.

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biomedical research

Cell-based therapy for modulating the immune response has gained momentum in recent years.

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science & patient

Both the Biotechnology and the Biomedical research conducted in our laboratory are aimed to advance and support the field of medicine.

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latest research

recent publications

Publications

  • S Sarkar, P Sabhachandani, T Konry,Ultrasensitive Isothermal Detection of Protein Analytes Using Rolling Circle Amplification in Microscale Platforms Rolling Circle Amplification (RCA), 85-97, 2016.
  • S. Sarkar, P. Sabhachandani, D. Stroopinsky, K. Palmer, N. Cohen, J. Rosenblatt, D. Avigan, T. Konry Dynamic analysis of immune and cancer cell interactions at single cell level in microfluidic droplets, Biomicrofluidics,1 (10), 704-709, 2016.
  • S Sarkar, P. Sabhachandani, T. Konry, Isothermal Amplification Strategies for Protein Detection in Microfluidic Devices, Trends in Biotechnology, 2016.
  • E. Hondroulis, A. Movila, P. Sabhachandani, S.Sarkar, N. Cohen, T.Kawai, T.Konry, A droplet- merging platform for comparative functional analysis of m1 and m2 macrophages in response to e. coli-induced stimuli, Biotechnology and Bioengineering, 10(5), 2016.
  • P. Sabhachandani, S. Sarkar, T.Konry, Droplet Microfluidics for Screening of Surface-Marker and Secretory Protein Expression, Book Chapter: Microfluidic Methods for Molecular Biology, 219-23, 2016.
  • L. Kahanovitz, E. Seker, R.S. Marks, M.L. Yarmush, T. Konry, S.J. Russell, Development of a Microsphere-Based System to Facilitate Real-Time Insulin Monitoring, Journal of diabetes science and technology, 3;10(3):689-96, 2016.
  • P. Sabhachandani, V. Motwani, N. Cohen, S. Sarkar, V. Torchilin, T. Konry, Generation and functional assessment of 3D multicellular spheroids in droplet based microfluidics platform, Lab Chip 2015.
  • T. Konry, S. Sarkar, P. Sabhachandani, N. Cohen, Innovative Tools and Technology for Analysis of Single Cells and Cell-Cell Interaction, Annual Review of Biomedical Engineering 18 (1), 2015.
  • S Sarkar, V Motwani, P Sabhachandani, N Cohen, T Konry, T Cell Dynamic Activation and Functional Analysis in Nanoliter Droplet Microarray, Journal of clinical & cellular immunology 6 (3),2015.
  • S. Sarkar, N. Cohen, P. Sabhachandania and T. Konry, Phenotypic drug profiling in droplet microfluidics for better targeting of drug-resistant tumors, Lab Chip,15, 4441-4450,2015.
  • Pooja Sabhachandani, Noa Cohen, Saheli Sarkar, Tania Korny, Microsphere-based immunoassay integrated with a microfluidic network to perform logic operations, Microchimica Acta, 182(9-10),2015.
  • Noa Cohen, Pooja Sabhachandani, Alexander Golberg, Tania Konry, Approaching near real-time biosensing: Microfluidic microsphere based biosensor for real-time analyte detection, Biosensors and Bioelectronics 66, 454, 2015.
  • T. Konry, A. Golberg, M. Yarmush, Live single cell functional phenotyping in droplet nano-liter reactors, Scientific Reports : Nature Publishing Group, 3, 3179, 2013.
  • T. Konry, Adam Lerner, Martin L. Yarmush, Irina V. Smolina, Target DNA detection and quantitation on a single cell with single base resolution, Technology, 01, 88, 2013.
  • A.Golberg, M. L. Yarmush, T. Konry, Pico-liter immunosorbent droplet microfluidic platform for point-of-care tetanus diagnostics, Microchimica Acta, 180, 9-10, 860, 2013.
  • G.Linshiz, A. Goldberg, T. Konry, N.J. Hillson, The fusion of biology, computer science, and engineering: towards efficient and successful synthetic biology, Perspectives in Biology and Medicine, 55 4, 503, 2012.
  • T. Konry et al., Particles and Microfluidic Merged: Perspective of highly Sensitive Diagnostic Detection, Microchemica Acta, 176, 3-4, 251, 2012.
  • T.Konry, I .Smolina, M.L. Yarmush, et al., Microfluidic nano-liter platform for ultrasensitive detection of low-abundance surface-marker protein using isothermal rolling circle amplification, Small, 7, 3, 395, 2011.
  • T. Konry, M. Dominguez, C. Baecher-Allan, M.Yarmush, Droplet-based microfluidic platforms for single T cell secretion analysis of IL -10 cytokine, Biosensors and bioelectronics, 26, 270, 2011. Top 20 Articles, in the Domain of Article 20888750.
  • T. Konry, D.R. Walt, “Intelligent medical diagnostics via molecular logic”. J. Am. Chem. Soc.,131 (37), 13232, 2009.
  • T. Konry, R. B. Hayman, D. R. Walt, Microsphere-based rolling circle amplification microarray for the detection of DNA and proteins in a single assay, Analytical Chemistry ,81(14), 5777, 2009.
  • T. Konry, S. Cosnier, K. Gorgy, R. S. Marks, Characterization of thin poly (Pyrrole-benzophenone) film morphologies electropolymerized on Indium Tin Oxide coated optic fibers for electrochemical and optical biosensing, Electrochimica Acta, 53, 5128, 2008.
  • T. Konry, B. Hadad, Y. Shemer-Avni ,S. Cosnier, R. S. Marks , ITO pattern fabrication of glass platforms for electropolymerization of light sensitive polymer for its conjugation to bioreceptors on a micro-array, Talanta, 75, 564, 2008.
  • T.Konry, M.Bouhirfd, M. Whelan, F. Rossi, R. S.Marks, Electrogenerated ITO-coated glass chip surfaces, Biosensors and bioelectronics, 22, 2230, 2007.
  • A.Petrosova, T. Konry, S.Cosnier, et al.,, Development of a highly sensitive, field operable biosensor to be deployed in central Africa for serological studies of Ebola virus, Sensors and Actuators, 122, 578, 2007.
  • T. Konry, A. Novoa, Y. et al.,, Optical fiber immunosensor based on poly(pyrrole-benzophenone) film for detection of antibodies to viral antigen, Analytical Chemistry, 77, 6, 1771, 2005.
  • T.Konry,A.Novoa,R.S.Marks, Physico-chemical studies of the properties of ITO-coated fiber-optic, Thin solid films, 492 313, 2005.
  • T.Konry, A.Novoa,S.Cosnier, R.S.Marks, Development of an ‘electroptode’ immunosensor: Indium tin oxide-coated optical fiber tips conjugated with an electro-polymerized thin film with conjugated cholera toxin B subunit, Analytical Chemistry, 75, 2633, 2003.

people

our lab

Master Students

Sai Mynampati, Abhishek Chiyyeadu, Sayalee Potdar, Sneha Pawar, Kristy Fang, Rucha Adhav, Chanchal Rathi, Himali Shroff, Chaitra Belgur, Dipen Parande

Undergraduate Students

Kristy Fang

Alumni

Vinny Motwani, Abhinav Gupta, Sneha Varghese, Dishant Patel, Harnil Shah, Noa Cohen, Micah AmdurClark

latest news

Dr.Konry was selected for funding by CIMIT’s NIH POC Award

The project is focused on developing a device for rapid urinary tract infection diagnosis and antibiotic susceptibility testing.

Our new findings in dynamic analysis of immune and cancer cell interactions: a single cell lab on a chip perspective

We investigated the dynamics of live cell anti-tumor immune responses at the single cell level in a microfluidic platform. The integrated droplet array allowed improved control over heterotypic cell pairing and interactions, which allowed us to observe significant cell motility, morphological changes, and complex formation over an extended duration. We evaluated immune cell priming by Ag-loaded DCs and the subsequent functional outcome in the con- text of multiple myeloma cells. Our results demonstrate substantial heterogeneity in priming interactions between DC and T cells, both in basal and activated cells. Effector T cells depicted time-varying cytotoxicity following transient, short or long stable contacts. Serial interactions by T cells were observed both in upstream (DC-based) and downstream (target-based) interactions. Our future aims include determining the molecular mechanisms underlying the phenotypic heterogeneity in T cell responses in droplets, and integrated live cell analysis of immune cell activation and effector functions.

Our novel droplet‐merging platform has been applied for comparative functional analysis of M1 and M2 macrophages for Forsyth institute

In our recent paper published in Biotechnology and Bioengineering we presented a simple and effective method for the co-encapsulation of polarized M1 and M2 macrophages with Escherichia coli (E. coli) by passive merging in an integrated droplet generation, merging, and docking platform. This approach facilitated live cell profiling of effector immune functions in situ and quantitative functional analysis of macrophage heterogeneity.

Our recent publication in Lab on a chip:

Phenotypic drug profiling in droplet microfluidics for better targeting of drug-resistant tumors

Acquired drug resistance is a key factor in the failure of chemotherapy. Due to intratumoral heterogeneity, cancer cells depict variations in intracellular drug uptake and efflux at the single cell level, which may not be detectable in bulk assays. In this study we present a droplet microfluidics-based approach to assess the dynamics of drug uptake, efflux and cytotoxicity in drug-sensitive and drug-resistant breast cancer cells. An integrated droplet generation and docking microarray was utilized to encapsulate single cells as well as homotypic cell aggregates. Drug-sensitive cells showed greater death in the presence or absence of Doxorubicin (Dox) compared to the drug-resistant cells. We observed heterogeneous Dox uptake in individual drug-sensitive cells while the drug-resistant cells showed uniformly low uptake and retention. Dox-resistant cells were classified into distinct subsets based on their efflux properties. Cells that showed longer retention of extracellular reagents also demonstrated maximal death. We further observed homotypic fusion of both cell types in droplets, which resulted in increased cell survival in the presence of high doses of Dox. Our results establish the applicability of this microfluidic platform for quantitative drug screening in single cells and multicellular interactions.

Dr. Konry was awarded with Schumacher Faculty Award 2015, presented to one faculty member early in their Northeastern career to recognize significant academic achievement for work done at Northeastern University

Dr. Konry was awarded with competitive grant from DFCI/NU Joint Program in Cancer Drug Development in collaboration with Prof. Suzanne Gaudet (DF/HMS).

Congratulations to Pooja Sabhachandani on being awarded with Shevell/Cohen Cancer Research Award (first place winner)

Our recent publication in Biosensors and Bioelectronics:

Approaching near real-time biosensing: Microfluidic microspherebased biosensor for real-time analyte detection

In this study we describe a simple lab-on-a-chip (LOC) biosensor approach utilizing well mixed micro- fluidic device and a microsphere-based assay capable of performing near real-time diagnostics of clinically relevant analytes such cytokines and antibodies. We were able to overcome the adsorption kinetics reaction rate-limiting mechanism, which is diffusion-controlled in standard immunoassays, by introducing the microsphere-based assay into well-mixed yet simple microfluidic device with turbulent flow profiles in the reaction regions. The integrated microsphere-based LOC device performs dynamic detection of the analyte in minimal amount of biological specimen by continuously sampling micro-liter volumes of sample per minute to detect dynamic changes in target analyte concentration. Furthermore we developed a mathematical model for the well-mixed reaction to describe the near real time detection mechanism observed in the developed LOC method. To demonstrate the specificity and sensitivity of the developed real time monitoring LOC approach, we applied the device for clinically relevant analytes: Tumor Necrosis Factor (TNF)alpha cytokine and its clinically used inhibitor, anti-TNF-α antibody. Based on the reported results herein, the developed LOC device provides continuous sensitive and specific near real-time monitoring method for analytes such as cytokines and antibodies, reduces reagent volumes by nearly three orders of magnitude as well as eliminates the washing steps required by standard immunoassays.

Our recent publication in PlosOne:

Cloud-Enabled Microscopy and Droplet Microfluidic Platform for Specific Detection of Escherichia coli in Water

Published: January 27, 2014 DOI: 10.1371/journal.pone.0086341

We report an all-in-one platform - ScanDrop - for the rapid and specific capture, detection, and identification of bacteria in drinking water. The ScanDrop platform integrates droplet microfluidics, a portable imaging system, and cloud-based control software and data storage. The cloud-based control software and data storage enables robotic image acquisition, remote image processing, and rapid data sharing. These features form a "cloud" network for water quality monitoring. We have demonstrated the capability of ScanDrop to perform water quality monitoring via the detection of an indicator coliform bacterium, Escherichia coli, in drinking water contaminated with feces. Magnetic beads conjugated with antibodies to E. coli antigen were used to selectively capture and isolate specific bacteria from water samples. The bead-captured bacteria were co-encapsulated in pico-liter droplets with fluorescently-labeled anti-E. coli antibodies, and imaged with an automated custom designed fluorescence microscope. The entire water quality diagnostic process required 8 hours from sample collection to online-accessible results compared with 2-4 days for other currently available standard detection methods.

contact us

Tania (Tali) Konry, Ph.D. Assistant Professor Department of Pharmaceutical Sciences Northeastern University 140 The Fenway, R 441, Lab 446 Boston, MA 02115 Tel: 617.373.3224