biotechnology research

The use of nano-liter reaction volumes and parallel sample processing offered by microfluidic devices make them ideally suited to total chemical and bioassay analysis, ultra-high throughput screening applications, and other cases where samples and reagents are available in limited quantities.

read more...

biomedical research

Cell-based therapy for modulating the immune response has gained momentum in recent years.

read more...

science & patient

Both the Biotechnology and the Biomedical research conducted in our laboratory are aimed to advance and support the field of medicine.

read more...

latest research

recent publications

Publications

  • Pooja Sabhachandani, Noa Cohen, Saheli Sarkar, Tania Korny, Microsphere-based immunoassay integrated with a microfluidic network to perform logic operations, Microchimica Acta, 2015, accepted.
  • Noa Cohen, Pooja Sabhachandani, Alexander Golberg, Tania Konry, Approaching near real-time biosensing: Microfluidic microsphere based biosensor for real-time analyte detection, Biosensors and Bioelectronics 66, 454, 2015.
  • T. Konry, A. Golberg, M. Yarmush, Live single cell functional phenotyping in droplet nano-liter reactors, Scientific Reports : Nature Publishing Group, 3, 3179, 2013.
  • T. Konry, Adam Lerner, Martin L. Yarmush, Irina V. Smolina, Target DNA detection and quantitation on a single cell with single base resolution, Technology, 01, 88, 2013.
  • A.Golberg, M. L. Yarmush, T. Konry, Pico-liter immunosorbent droplet microfluidic platform for point-of-care tetanus diagnostics, Microchimica Acta, 180, 9-10, 860, 2013.
  • G.Linshiz, A. Goldberg, T. Konry, N.J. Hillson, The fusion of biology, computer science, and engineering: towards efficient and successful synthetic biology, Perspectives in Biology and Medicine, 55 4, 503, 2012.
  • T. Konry et al., Particles and Microfluidic Merged: Perspective of highly Sensitive Diagnostic Detection, Microchemica Acta, 176, 3-4, 251, 2012.
  • T.Konry, I .Smolina, M.L. Yarmush, et al., Microfluidic nano-liter platform for ultrasensitive detection of low-abundance surface-marker protein using isothermal rolling circle amplification, Small, 7, 3, 395, 2011.
  • T. Konry, M. Dominguez, C. Baecher-Allan, M.Yarmush, Droplet-based microfluidic platforms for single T cell secretion analysis of IL -10 cytokine, Biosensors and bioelectronics, 26, 270, 2011. Top 20 Articles, in the Domain of Article 20888750.
  • T. Konry, D.R. Walt, “Intelligent medical diagnostics via molecular logic”. J. Am. Chem. Soc.,131 (37), 13232, 2009.
  • T. Konry, R. B. Hayman, D. R. Walt, Microsphere-based rolling circle amplification microarray for the detection of DNA and proteins in a single assay, Analytical Chemistry ,81(14), 5777, 2009.
  • T. Konry, S. Cosnier, K. Gorgy, R. S. Marks, Characterization of thin poly (Pyrrole-benzophenone) film morphologies electropolymerized on Indium Tin Oxide coated optic fibers for electrochemical and optical biosensing, Electrochimica Acta, 53, 5128, 2008.
  • T. Konry, B. Hadad, Y. Shemer-Avni ,S. Cosnier, R. S. Marks , ITO pattern fabrication of glass platforms for electropolymerization of light sensitive polymer for its conjugation to bioreceptors on a micro-array, Talanta, 75, 564, 2008.
  • T.Konry, M.Bouhirfd, M. Whelan, F. Rossi, R. S.Marks, Electrogenerated ITO-coated glass chip surfaces, Biosensors and bioelectronics, 22, 2230, 2007.
  • A.Petrosova, T. Konry, S.Cosnier, et al.,, Development of a highly sensitive, field operable biosensor to be deployed in central Africa for serological studies of Ebola virus, Sensors and Actuators, 122, 578, 2007.
  • T. Konry, A. Novoa, Y. et al.,, Optical fiber immunosensor based on poly(pyrrole-benzophenone) film for detection of antibodies to viral antigen, Analytical Chemistry, 77, 6, 1771, 2005.
  • T.Konry,A.Novoa,R.S.Marks, Physico-chemical studies of the properties of ITO-coated fiber-optic, Thin solid films, 492 313, 2005.
  • T.Konry, A.Novoa,S.Cosnier, R.S.Marks, Development of an ‘electroptode’ immunosensor: Indium tin oxide-coated optical fiber tips conjugated with an electro-polymerized thin film with conjugated cholera toxin B subunit, Analytical Chemistry, 75, 2633, 2003.

people

our lab

Master Students

Vinny Motwani, Abhinav Gupta, Sneha Varghese, Dishant Patel, Harnil Shah

Undergraduate Students

Micah AmdurClark

latest news

Congratulations to Pooja Sabhachandani on being awarded with Shevell/Cohen Cancer Research Award (first place winner)

Our recent publication in Biosensors and Bioelectronics:

Approaching near real-time biosensing: Microfluidic microspherebased biosensor for real-time analyte detection

In this study we describe a simple lab-on-a-chip (LOC) biosensor approach utilizing well mixed micro- fluidic device and a microsphere-based assay capable of performing near real-time diagnostics of clinically relevant analytes such cytokines and antibodies. We were able to overcome the adsorption kinetics reaction rate-limiting mechanism, which is diffusion-controlled in standard immunoassays, by introducing the microsphere-based assay into well-mixed yet simple microfluidic device with turbulent flow profiles in the reaction regions. The integrated microsphere-based LOC device performs dynamic detection of the analyte in minimal amount of biological specimen by continuously sampling micro-liter volumes of sample per minute to detect dynamic changes in target analyte concentration. Furthermore we developed a mathematical model for the well-mixed reaction to describe the near real time detection mechanism observed in the developed LOC method. To demonstrate the specificity and sensitivity of the developed real time monitoring LOC approach, we applied the device for clinically relevant analytes: Tumor Necrosis Factor (TNF)alpha cytokine and its clinically used inhibitor, anti-TNF-α antibody. Based on the reported results herein, the developed LOC device provides continuous sensitive and specific near real-time monitoring method for analytes such as cytokines and antibodies, reduces reagent volumes by nearly three orders of magnitude as well as eliminates the washing steps required by standard immunoassays.

Our recent publication in PlosOne:

Cloud-Enabled Microscopy and Droplet Microfluidic Platform for Specific Detection of Escherichia coli in Water

Published: January 27, 2014 DOI: 10.1371/journal.pone.0086341

We report an all-in-one platform - ScanDrop - for the rapid and specific capture, detection, and identification of bacteria in drinking water. The ScanDrop platform integrates droplet microfluidics, a portable imaging system, and cloud-based control software and data storage. The cloud-based control software and data storage enables robotic image acquisition, remote image processing, and rapid data sharing. These features form a "cloud" network for water quality monitoring. We have demonstrated the capability of ScanDrop to perform water quality monitoring via the detection of an indicator coliform bacterium, Escherichia coli, in drinking water contaminated with feces. Magnetic beads conjugated with antibodies to E. coli antigen were used to selectively capture and isolate specific bacteria from water samples. The bead-captured bacteria were co-encapsulated in pico-liter droplets with fluorescently-labeled anti-E. coli antibodies, and imaged with an automated custom designed fluorescence microscope. The entire water quality diagnostic process required 8 hours from sample collection to online-accessible results compared with 2-4 days for other currently available standard detection methods.

contact us

Tania (Tali) Konry, Ph.D. Assistant Professor Department of Pharmaceutical Sciences Northeastern University 140 The Fenway, R 441, Lab 446 Boston, MA 02115 Tel: 617.373.3224