Presentations and Publications
Examples of Recent Presentations and Publications by ECRP Staff
Shimelis, O., Zhou, X., Li, G., Giese, R. W. (2004) Phenolic extraction of DNA from mammalian tissues and conversion to deoxyribonucleoside-5'-monophosphates devoid of ribonucleotides, J. Chromatogr. A., 1053, 143-149.
Shao, G., Giese. R. W. (2004) Trace Detection of Glycolic Acid by Electrophore Labeling Gas Chromatography-Electron Capture Mass Spectrometry, Anal. Chem. 76, 3049-3054.
Kautz, R. A., Karger, B.L. Method of Efficient Transport of Small Liquid Volumes To, From, or Within Microfluidic Devices, Patent App WO/2005/059512, PCT/US2004/041923.
Riciciki, E. M., Soglia, J. R., Teitel, C., Kane, R., Kadlubar, F., Vouros, P. (2005) Detection and Quantification of N-(deoxyguanosine-8-yl)-4-aminobiphenyl Adducts in Human Pancreas Tissue Using Capillary Liquid Chromatography-Microelectrospray Mass Spectrometry, Chem. Res. Toxicol. 18, 692-699.
Kao, C-Y., Giese, R.W. (2005) Measurement of N7-(2'-Hydroxyethyl)guanine in Human DNA by Gas Chromatography Electron Capture Mass Spectrometry, Chem. Res. Toxicol. 18, 70-75.
Kautz, R. A., Goetzinger, W., and Karger, B.L. (2005) High-Throughput Microcoil NMR of Compound Libraries Using Zero-Dispersion Segmented Flow Analysis. J. Comb. Chem. 7, 14-20.
Flarakos, J., Xiong, W., Glick, J., Vouros, P. (2005) Deoxynucleotide Derivatization Methodology for Improving LC-ESI-MS Detection, Anal Chem. 77, 2373-2380.
Giese, R. W., "Mass Specific Discovery of DNA Adducts", Lombardi Cancer Center, George Washington University, April 6, 2005; Capitol University of Medical Sciences, Beijing, China, Nov.1, 2005.
Beuning, P. J., "Critical Role of Processivity Clamp Proteins in the Cellular Response to DNA Damage", seminar, Department of Civil Environmental Engineering, Northeastern University, November 10, 2006.
Levin, D. S., Vouros, P., Miller, R. A., Nazarov, E. G., Morris, J. C. (2006) Characterization of gas phase interactions utilizing a novel nanoelectrospray ionization-differential mobility-mass spectrometer (nanoESI0DMS-MS) system, Anal. Chem. 78, 96-106.
Murugaiah, V., Naim, A., Peng, S., Cui, G., Giese, R. W. (2006) 4-(4'-Methyltetrafluorophenyl)-2,3,5,6-tetrafluorobenzyl bromide: A new electrophoric derivatizing reagent, J. Chromatogr. A, 1134, 338-340.
Riciciki, E. M., Andrews, C. L., Schiavo, S., Vouros, P. (2006) Analysis of DNA Adducts, in Encyclopedia of Mass Spectrometry, vol 3, Biological Applications Part B: Carbohydrates, Nucleic Acids and other Biological compounds, Series editors: Caprioli, R., and Gross, M. Elsevier Ltd., Oxford, 61-85.
Riciciki, E.M., Luo, W., Zarbl, H., Vouros, P. (2006) "Quantification of N-(deoxyguanosin-8-yl)-4-aminobiphenyl Adducts in Human Lymphoblastoid TK6 Cells Dosed with N-hydroxy-4-aminobiphenyl and their Relationship to Mutation, Toxicity and Gene Expression Profiling, Anal. Chem.78, 6422-6432.
Beuning, P. J., Simon, S, M, Zemla, A., Barsky, D., Walker G. C. (2006) A Non-Cleavable UmuD Variant that Acts as a UmuD Mimic, J. Biol. Chem. 281, 9633-9640.
Beuning P. J., Sawicka, D., Barsky, D., Walker, G. C. (2006) Two Processivity Clamp Interactions Differentially Alter the Dual Activities of UmuC, Molecular Microbiology 59 460-474.
Beuning, P. J., Simon, S. M., Godoy, V. G., Jarosz, D. F., Walker G. C. (2006) Characterization of E. coli translesion synthesis polymerases and their accessory factors, Methods in Enzymology 408, 318-340.
Cruceanu, M., Stephen, A. G., Beuning, P.J., Gorelick, R. J., Fisher, R. J., Williams, R. J. (2006) Single DNA molecule stretching experiments provide an efficient method for determining the activity of drugs that target the HIV-1 nucleocapsid protein, Analytical Biochemistry 358 159-170.
Shimelis, O., Giese, R. W. (2006) Nuclease P1 digestion/high-performance liquid chromatography, a practical method for DNA quantitation, J. Chromatogr. A. 1117, 132-136.
Vorous, P., Greater Montreal Mass Spectrometry Discussion Group, "Monitoring DNA Damage from Environmental Carcinogens by Capillary LC-MS", Jan. 26, 2006.
Giese, R. W., "Measurement and meaning of DNA Adducts", University of New Hampshire, Durham, NH, Sept. 28, 2006.
Liao Q. Chiu N. H. L., Shen C., Vouros, P. (2007) Investigation of the Enzymatic Behavior of Benzonase Alkaline Phosphatase in the Digestion of DNA and DNA Adducts by ESI-LC/MS, Anal. Chem. 79, 1907-1917.
Lin Y, Vouros P, Orjala J., Kautz R. A. (2007) An Ultrasensitive LC-MS-NMR Platform Utilizing Segmented-Flow Microcoil NMR, Nanospray ESI, and 4 mm LC Column, invited lecture, 48th Experimental NMR Conference, Daytona FL, 26 April 2007.
Sefcikova, J. Beuning, P. J., "Substrate specificity of DNA polymerases that Replicate Damaged DNA", American Chemical Society 2007 Fall Meeting, Boston, MA August 19-23, 2007.
Yong. L. C., Schulte, P. A., Kao, C-K., Giese, R. W., Boeniger, M. F., Strauss, G. H.S., Petersen, M. R., Wiencke, J. K. (2007) DNA Adducts in Granulocytes of Hospital Workers Exposed to Ethylene Oxide, Am. J. Ind. Med., 50, 293-302.09.
Beuning P. J., "A Single Point Mutation in E. coli DNA Polymerase V Renders Cells Hypersensitive to UV Light", seminar, Institute of Biotechnology, Vilnius, Lithuania, September 21, 2007.
Jarosz, D. F., Beuning, P. J., Cohen, S. E., Walker, G. C. (2007) Y-Family DNA Polymerase in Escherichia coli, Trends in Microbiol. 15, 70-77.
Xiong, W.; Glick, J.; Lin, Y., Vouros, P. (2007) Separation and Characterization of Isomeric Oligonucleotide Adducts Using Monolithic Columns by Ion-pair Reversed-Phase Nano- HPLC Coupled to Ion Trap Mass Spectrometry, Anal. Chem. 79, 5312-5321.
Yong. L. C., Schulte, P. A., Kao, C-K., Boeniger, M. F., Strauss, G. H.S., Petersen, M. R., Wiencke, J. K., Giese, R. W. (2007) DNA Adducts in Granulocytes of Hospital Workers Exposed to Ethylene Oxide, Am. J. Ind. Med., 50, 293-302.
Shao, G., Kautz, R. A., Peng, S., Cui,G., Giese, R.W. (2007) Calibration by NMR for quantitative analysis: p-Toluenesulfonic Acid as an Internal Standard, J. Chromatogr. A 1138, 305-308.
Kautz, R. A. "An Optimized LC-MS+NMR Strategy Using a Microcoil NMR Probe with Normal-bore LC," invited presentation, NIH/NIA Baltimore, MD, 23 April 2008.
Micah J. McCauley, Leila Shokri, Jana Sefcikova, Česlovas Venclovas, Penny J. Beuning, and Mark C. Williams (2008) “Distinct Double- and Single-Stranded DNA Binding of E. coli Replicative DNA Polymerase III Alpha Subunit” ACS Chemical Biology 3 577-587. Featured in journal Podcast, September 2008: http://community.acs.org/ChemBiol/Podcasts/2008/tabid/110/Default.aspx.
Schiavo S, Orjala J, Vouros P, Kautz R. (2008) Microscale LC-MS-NMR platform applied to the identification of active cyanobacterial metabolites.
McCauley, M., Shokri, L., Venclovas, Beuning, P. J., Williams, M. C., (2008) "Double and Single Strand DNA Binding of Catalytic Alpha Subunit of E. Coli Replicative DNA Polymerase III", Biophysical Society of Annual Meeting, Long Beach, CA, February 2-6. Beuning, P. J., Shurtleff, B., Sefcikova, J., Walker G. C., Steric Gate Variants in a Y family DNA Polymerase Confer UV-Hypersensitivity American Society for Biochemistry and Molecular Biology (Experimental Biology 2009), San Diego, CA, April 5-9, 2008. Giese, R., "Genotoxin Mass Profiling for Environmental Regulation", Department of Environmental Protection, Boston, January 22, 2008. Giese, R. "Mass Tag Profiling of Chemical Damage to DNA", Pfizer, Groton, CT, April 16, 2008; Genotoxic Impurities Conference, Brussels, Belgium, June 17, 2008. Xiong, W., Glick, J., Lin, Y., Noronha, A.M., Wilds, C.J., and Vouros, P. “The Influence of Cytosine Methylation on the Chemoselectivity of Benzo[a]pyrene Diol Epoxide-Oligonucleotide Adducts Determined Using nano LC-MS/MS”,Journal of Mass Spectrom. 44, 1241-1248 (2009).
Liao, Q., Shen, C., and Vouros, P., "GenoMass - A Computer Software for Automated Identification of Oligonucleotide DNA Adducts from LC-MS Analysis of DNA Digests", Journal of Mass Spectrom., 44, 549-560 (2009).
Zarbl, H.; Gallo, M. A.; Glick, J., Yeung, K. Y.; Vouros, P., “The Vanishing Zero Revisited: Thresholds in the Age of Genomics”, Chemico-Biological Interactions, 184, 273-278 (2010).
Liao, Q. And Vouros, P., “Oligonucleotide Adducts as Markers for Site Selective Carcinogen Adduction: Their analysis by Mass Spectrometry Coupled to Separation Methods”, Advances In Chromatography Series, Vol. 49, 187-249 (2010).
Ding, Y., Paonessa, J.D., Randall, K.L., Argoti, D., Chen, L., Vouros, P., Zhang, Y., Sulforaphane inhibits 4-Aminobiphenyl-induced DNA Damage in Bladder Cells and Tissues” Carcinogenesis, 31, 199-2003 (2010).
Randall, K. L., Argoti, D., Paonessa, J. D., Ding, Y., Oaks, Z., Zhang, Y., Vouros, P., “An Improved Liquid Chromatography-Tandem Mass Spectrometry Method for the Quantification of 4-Aminobiphenyl DNA Adducts in Urinary Bladder Cells and Tissues”, Journal of Chromatography A , 1217, 4135-4143 (2010) PMID: 19932483.
Jing Fang, Kasper D. Rand, Michelle C. Silva, Thomas E. Wales, John R. Engen, and Penny J. Beuning (2010) “Conformational Dynamics of the Escherichia coli polymerase manager proteins UmuD and UmuD′ ” Journal of Molecular Biology 398 40-53.
Jaylene N. Ollivierre*, Jing Fang*, and Penny J. Beuning (2010) “The roles of UmuD in regulating mutagenesis” Journal of Nucleic Acids Article ID 947680, 12 pages, 2010. doi:10.4061/2010/947680.
Vaneet K. Sharma, James Glick, Paul Vouros (2012) Reversed-phase ion-pair liquid chromatography electrospray ionization tandem mass spectrometry for separation, sequencing and mapping of sites of base modification of isomeric oligonucleotide adducts using monolithic column, J. Chromatogr. 1245,65–74.
Klaene J. J., Sharma V.K., Glick J, Vouros P. (2012) The analysis of DNA adducts: The transition from (32)P-postlabeling to mass spectrometry, Cancer Lett. doi:pii: S0304-3835(12)00478-8. 10.1016/j.canlet.2012.08.007. [Epub ahead of print] PMID:22960573.
Ollivierre, J. N., Sikora, J. L., Beuning, P. J. (2013) Dimer exchange and cleavage specificity of the DNA damage response protein UmuD, Biochimica et Biophysica Acta-Proteins and Proteomics 1834 611-620.