John R. Engen

Full Professor

140 The Fenway
Tel: 617.373.6046
Fax: 617.373.2855
j.engen@neu.edu
Engen Laboratory Website

Bioanalytical Chemistry and Mass Spectrometry

Education:

1994 B.S., Molecular Biology, Union College
1995 B.S., Biochemistry, Union College
1999 Ph.D., Analytical Chemistry, University of Nebraska-Lincoln

Awards

2009 Arthur F. Findeis Award from the American Chemical Society

Research Interests

Current research projects in his laboratory include (1) investigations of kinase conformation to understand regulation and aberrant signaling in various disease states including cancer, (2) analysis of the conformation of viral accessory proteins from HIV, (3) studies of protein conformation at biological membranes, and (4) optimization and methods development in hydrogen exchange mass spectrometry.

Selected Publications

Full publications list here, or see laboratory website.

Kim, M., Sun, Z.-Y. J., Rand, K. D., Shi, X., Song, L., Cheng, Y., Fahmy, A. F., Majumdar, S., Ofek, G., Yang, Y., Kwong, P. D., Wang, J.-H., Engen, J. R., Wagner, G. & Reinherz, E. L. (2011). Antibody mechanics on a membrane-bound HIV segment essential for gp41-targeted viral neutralization. Nat. Struct. Mol. Biol. 18(11), 1235-1243. Pubmed: 22002224.

Morgan, C. R., Hebling, C. M., Rand, K. D., Stafford, D. W., Jorgenson, J. W. & Engen, J. R. (2011). Conformational transitions in the membrane scaffold protein of phospholipid bilayer nanodiscs. Mol. Cell. Proteomics 10(9), M111.010876. Pubmed: 21715319.

Marcsisin, S. R., Narute, P. S., Emert-Sedlak, L. A., Kloczewiak, M., Smithgall, T. E. & Engen, J. R. (2011). On the solution conformation and dynamics of the HIV-1 Viral Infectivity Factor. J. Mol. Biol. 410(5), 1008-1022. Pubmed: 21763503.

Houde, D. Berkowitz, S. A. & Engen, J. R. (2011). The utility of Hydrogen/Deuterium exchange mass spectrometry in biopharmaceutical comparability studies. J. Pharm. Sci. 100(6), 2071-2086. Pubmed: 21491437.

Tiyanont, K., Wales, T. E., Aste-Amezaga, M., Aster, J. C., Engen, J. R. & Blacklow, S. C. (2011). Evidence for increased exposure of the Notch1 metalloprotease cleavage site upon conversion to the activated conformation. Structure 19(4), 546-554. Pubmed: 21481777.

Kent, M. S., Murton, J. K., Sasaki, D. Y., Satija, S., Akgun, B., Nanda, H., Curtis, J. E., Majewski, J., Morgan, C. R. & Engen, J. R. (2010). Neutron reflectometry study of the conformation of HIV Nef bound to lipid membranes. Biophys. J. 99(6), 1940-1948. Pubmed: 20858440.

Hebling, C. M., Morgan, C. R., Stafford, D. W., Jorgenson, J. W., Rand, K. D. & Engen, J. R. (2010). Conformational analysis of membrane proteins in phospholipid bilayer nanodiscs by hydrogen exchange mass spectrometry. Anal. Chem. 82(13), 5415-5419. Pubmed: 20518534.

Zhang, J., Adrián, F. J., Jahnke, W., Cowan-Jacob, S. W., Li, A. G., Iacob, R. E., Sim, T., Powers, J., Dierks, C., Sun, F., Guo, G.-R., Ding, Q., Okram, B., Choi, Y., Wojciechowski, A., Deng, X., Liu, G., Fendrich, G., Strauss, A., Vajpai, N., Grzesiek, S., Tuntland, T., Liu, Y., Bursulaya, B., Azam, M., Manley, P. W., Engen, J. R., Daley, G. Q., Warmuth, M. & Gray, N. S. (2010). Targeting Bcr-Abl by combining allosteric with ATP-binding-site inhibitors. Nature 463(7280), 501-506. Pubmed: 20072125.

Engen, J. R. (2009). Analysis of protein conformation and dynamics by Hydrogen/Deuterium exchange mass spectrometry. Anal. Chem. 81(19), 7870-7875. Pubmed: 19788312. Featured on the cover.

Iacob, R. E., Dumitrescu, T. P., Zhang, J., Gray, N. S., Smithgall, T. E. & Engen, J. R. (2009). Conformational disturbance in Abl kinase upon mutation and deregulation. Proc. Natl. Acad. Sci. U.S.A. 106(5), 1386-1391. Pubmed: 19164531.

Wales, T. E & Engen, J. R. (2006). Hydrogen exchange mass spectrometry for the analysis of protein dynamics. Mass Spectrom. Rev. 25(1), 158-170. Pubmed: 16208684.