Physico-Chemical Nanocharacterization (PCNC)
Core Leader: Professor Srinivas Sridhar
PCNC Manager: Dr. Dattatri Nagesha
Electron Microscopy Coordinator: William Fowle
The PCNC provides the necessary physico-chemical characterization of nanopreparations in all the CCNE supported projects. The PCNC has the following aims:
- Characterize nanopreparations developed by Center investigators with respect to Size distribution, Stability and Storage conditions, adsorption of foreign matter , in buffer and blood plasma.
- Characterize the morphology of nanopreparations through high resolution microscopies including Scanning Electron Microscopy, Transmission Electron Microscopy and Atomic Force Microscopy
- Characterize the drug loading and drug release in buffer and blood through optical fluorescence and ultraviolet spectroscopy measurement
- Provide feedback to Center investigators to refine formulations, establish a database of characteristics, and forward optimized formulations to the Scaling Up and Manufacturing Core.
- Transmission Electron Microscopy (TEM) is available for use to analyze the morphology, size and size distribution of the prepared nanoformulations. Ultrathin sectioning is also possible to analyze interaction of nanoformulations with cells and tissues.
- Scanning Electron Microscopy (SEM) is available for use to analyze the morphology, size and size distribution of the prepared nanoformulations. Energy dispersive X-ray analysis is available for the elemental analysis or chemical characterization of a sample.
- Atomic Force microscopy (AFM Agilent – 5500 Atomic Force Microscope (AFM) with capabilities for topography imaging, scanning tunneling microscopy, phase imaging, electric force microscopy, magnetic force microscopy, MAC mode, contact mode, force modulation, acoustic AC mode and imaging in liquid medium.
Particle Size Analysis and Zeta Potential:
- Particle Size Analyzer 90 Plus from Brookhaven Instruments Company. This equipment contains a high power 35Mw diode laser for measurements of particle in the range of 1nm-6µm. The accessory BI-PALS can measure Zeta potential in organic solvents, in viscous media and in high-salt suspensions. The PCNC will assist in the particle size and zeta potential measurements of all the nanopreparations from the various projects.
- Drug Loading and Release Studies The PCNC also has capabilities to carry out drug loading and releases studies from various nanopreparations. This can be monitored using Agilent 8453 UV-Visible spectrophotometer or the Horiba FluoroMax – Compact Spectrofluorometer. We also have a Custom Fluorescence Spectrometer for Release Studies. The protocols for using these techniques will be tailored for each project and developed as a collaborative effort with the respective project PI.
This core brings expertise in the basic science and engineering aspects of nanotechnology that is essential to understand the fundamental properties of these nanopreparations.