| Roger W. Giese|
Professor of Chemistry and Biomedical Science; Director, Environmental Cancer Research Program; Senior Faculty Fellow, Barnett Institute; Editor, Journal of Chromatography
Department: Department of Pharmaceutical Sciences
Department 2: School of Pharmacy
Office: 140 TF, Room 202
Certification: Diplomate, American Board of Clinical Chemistry
The research projects in Dr. Giese's laboratory combine aspects of analytical chemistry, organic chemistry and biochemistry to make advances in the reagents and techniques available for trace organic analysis. Emphasis is placed on the development of new methodology for high sensitivity and multi-analyte detection, applied especially to human samples. The principle detection techniques being used for high sensitivity are mass spectrometry and capillary electrophoresis with laser-induced fluorescence detection. Examples of analytes of interest are DNA adducts (damage to genes by carcinogens) and steriods. Potentially some of these measurements may help to individualize cancer preventions.
Zhang, X., Linxiao, X., Wang, P., Wang, Z., Giese, R.W. Synthesis of Releasable Electrophore Tags for Applications in Mass Spectrometry, Bioconjugate Chemistry13, 1002-1012 (2002)
Li, G., Gao, J., Zhou, X., Shimelis, O., Giese, R.W. Handling and detection of 25 amol of near-infrared dye deoxynucleotide conjugates by capillary electrophoresis with laser-induced flourescence detection, J. Chromatogr. A, 1004, 47-50 (2003)
Shimelis, O., Zhou, X., Li, G., Giese, R.W. Phenolic extraction of DNA from mammalian tissues and conversion to deoxyribonucleoside-5?-monophosphates devoid of irbonucleotides, J. Chromatogr.A., 1053, 143-149 (2004)
Kao, C-Y., Giese, R.W. Measurement of N7-(2?-Hydrexyethyl)guanine in Human DNA by Gas Chromatography Electron Capture Mass Spectrometry, Chem. Res. Toxicol. 18, 70-75 (2005)
Giese, R. W. (2003) Measurement of endogenous estrogens: analytical challenges and recent advances, J. Chromatogr. A, 1000, 401-412.
Murugaiah, V., Naim, A., Peng, S., Cui, G., Giese, R. W. (2006) 4-(4'-Methyltetrafluorophenyl)-2,3,5,6-tetrafluorobenzyl bromide: A new electrophoric derivatizing reagent, J. Chromatogr. A, 1134, 338-340.
Yong. L. C., Schulte, P. A., Kao, C-K., Giese, R. W., Boeniger, M. F., Strauss, G. H.S., Petersen, M. R., Wiencke, J. K. (2007) DNA Adducts in Granulocytes of Hospital Workers Exposed to Ethylene Oxide, Am. J. Ind. Med., 50, 293-302.09.