John R. Engen
Bioanalytical Mass Spectrometry
The Barnett Institute
The rate of HX depends on hydrogen bonding and solvent accessibility. Folded proteins can have amino acids with HX rates as much as 1 billion times slower than the same amino acid that is not in a folded protein. Protein folding and unfolding, whether in cells or in the test tube, represent large changes in protein structure, hydrogen bonding and solvent accessibility that can be investigated with HX MS. Smaller structural changes critical for protein function can also be probed with HX MS.
There are ongoing opportunities for undergraduate, graduate and postdoctoral research in the group. Interested people should contact Prof. Engen by email.
Selected Recent Publications
Houde, D., Arndt, J., Domeier, W., Berkowitz, S. & Engen, J. R. (2009). Characterization of IgG1 conformation and conformational dynamics by hydrogen/deuterium exchange mass spectrometry. Anal. Chem. 81(7), 2644-2651.
Iacob, R. E., Dumitrescu, T. P., Zhang, J., Gray, N. S., Smithgall, T. E. & Engen, J. R. (2009). Conformational disturbance in Abl kinase upon mutation and deregulation. Proc. Natl. Acad. Sci. U.S.A. 106(5), 1386-1391.
Iacob, R. E., Murphy, J. P. 3rd & Engen, J. R. (2008). Ion mobility adds an additional dimension to mass spectrometry analysis of solution-phase hydrogen/deuterium exchange. Rapid Commun. Mass Spectrom. 22(18), 2898-2904.
Chen, S., Dumitrescu, T. P., Smithgall, T. E. & Engen, J. R. (2008). Abl N-terminal Cap stabilization of SH3 domain dynamics. Biochemistry 47(21), 5795-5803.
Wales, T. E., Fadgen, K. E., Gerhardt, G. C. & Engen, J. R. (2008). High-speed and high-resolution UPLC separation at zero degrees Celsius. Anal. Chem. 80(17), 6815-6820.
Engen, J. R., Wales, T. E., Hochrein, J. M., Meyn, M. A., Ozkam, S. B., Bahar, I. & Smithgall, T. E. (2008). Structure and dynamic regulation of Src-family kinases. Cell. Mol. Life Sci. 65(19), 3058-3073.
Mitchell, J. L. Trible, R. P., Emert-Sedlak, L. A., Weis, D. D., Lerner, E. C., Applen, J. J., Sefton, B. M., Smithgall, T. E. & Engen, J. R. (2007). Functional characterization and conformational analysis of the Herpesvirus saimiri Tip-C484 protein. J. Mol. Biol. 366(4), 1282-1293.
Trible, R. P., Emert-Sedlak, L., Wales, T. E., Ayyavoo, V., Engen, J. R., & Smithgall, T. E. (2007). Allosteric loss-of-function mutations in HIV-1 Nef from a Long-term Non-progressor. J. Mol. Biol. 374(1), 121-129.
Wales, T. E & Engen, J. R. (2006). Hydrogen exchange mass spectrometry for the analysis of protein dynamics. Mass Spectrom. Rev. 25(1), 158-170.