My project is investigating the role of cellular proteases in persister formation in Mycobacterium tuberculosis (MTb) and other Gram-positive pathogens. It’s previously been shown that persisters may form through the action of toxins, which must be freed from their cognate antitoxin by degradation. Proteases may also contribute to antibiotic survival by degrading drug targets. In order to determine whether these genes affect persister formation, I have created over-expression and knockout strains in MTb and other Gram-positives. These strains are then tested to determine how their survival rate compares to their parent. If the Clp protease complex is shown to be necessary for persister formation, it would present an appealing target for new drugs to treat MTb and other complex infections.